Table 1.

Agreement of the PLBL2 level (µg/mL) measured by DIA SWATH MS Top 3 approach with that by PLBL2 specific ELISA in HCCF from following expression conditions: CHO host cell lines 1, 2, and 3, all harvested on Day 14; Host cell line 3, harvested on Day 10 and 17. The results were the average of 2 biological replicates. For each biological replicate sample, PLBL2 concentrations were averages of four replicate analyses. Mean RSD (of five samples) was 5.0%, with highest RSD observed at 9.4%.

Sample (n = 2)	By SWATH Top 3 (μg/mL)	By Specific ELISAa (μg/mL)
Host 1-Day 14	7.7 ± 1.5	7.6
Host 2-Day 14	4.1 ± 0.35	6.3
Host 3-Day 14	2.1 ± 0.18	1.5
Host 3-Day 10	2.3 ± 0.04	1.6
Host 3-Day 17	1.6 ± 0.04	1.6

^aReference²⁵.