Fig. 1.

Effect of G17 on MAPK activation and migration in gastric cancer cells. A, Confluent AGSE cells were wounded linearly using a small pipette tip followed by treatment in the absence (−) or presence (+) of 100 nm G17. Phase-contrast microscopic pictures of the migratory cells were obtained at the indicated time points using inverted phase-contrast microscopy (Axiovert 200 inverted microscope; Zeiss), interfaced with a camera (Axiocam). B, AGSE cells treated as in panel A were harvested at different time points, and total protein was extracted. Western blot analyses of the cell extracts were then performed with the indicated antibodies.