Fig. 4.

Effect of modulation of JNK1 pathway on G17-induced migration. A, Subconfluent AGSE cells were transiently transfected with an empty vector, a JNK1-WT vector, a JNK1-CA vector, or JNK1-DN vector. The cells were wounded 48 h post-transfection and, following an overnight recovery after wounding, they were incubated with G17 and pictures were obtained at the indicated times. B, Subconfluent AGSE cells were transiently transfected with 100 nm of either control-siRNA or JNK1-siRNA, followed by wounding and G17 treatment after 48 h. C, Western blot analysis of AGSE cells transfected with either control-siRNA or JNK1-siRNA, followed by G17 treatment for 30 min. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.