Fig. 3.

Temporo-spatial loading of GR to the FKBP51 locus in response to glucocorticoid exposure in A549 cells. A, Cells were treated with 100 nm dex for indicated times, and ChIPs with anti-GR antibody followed by real-time PCR were carried out as described in Materials and Methods to investigate the occupancy of GR within 17 different regions spanning approximately 160 kb of chromosome 6 that covers the FKBP51 locus plus its surroundings (cf. Fig. 2A). A region in the middle of DSCAM in chromosome 22 served as a nontarget control. ChIP assays with normal IgG monitored nonspecific binding. Results are shown as percentages of the input samples. Columns represent the mean ± sd of three experiments. B, Binding of GR to the FKBP51 regions −3, 8, 9, and 10 plotted against time after addition of the hormone. Ctrl., Control.