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. 2010 Mar;24(3):511–525. doi: 10.1210/me.2009-0443

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Fig. 6. — Evidence for the involvement of SWI/SNF type chromatin-remodeling complexes in the regulation of the FKBP51 locus. The effect of glucocorticoid on the occupancy of BRM- or BRG1-containing complexes within the FKBP51 locus was analyzed in A549 cells treated with vehicle or dex as in Fig. 4 by ChIP assays using antibodies against BRM (A) or BRG1 (B) followed by QPCR analysis. Results are shown as fold over the normal rabbit IgG-precipitated samples and represent the mean ± sd of three experiments. C, Depletion of BRM from A549 cells transfected with BRM-specific siRNA as assessed by immunoblotting. The level of glyceraldehyde-3-phosphate dehydrogenase monitored the loading of protein in the samples. D, The effect of BRM siRNA on the glucocorticoid induction of FKBP51 mRNA as analyzed by RT-QPCR analysis. ***, P < 0.001 compared with the cells transfected with unspecific target siRNA (siSCR) and treated with dex. GAPDH, Glyceraldehyde-3-phosphate dehydrogenase.