Fig. 6.
E2-induced reduction of KATP channel activity is abolished in β-cells from GC-A KO mice. A, E2 1 nm decreased KATP channel activity in intact pancreatic islet cells from WT mice. The records show KATP channel activity before application of E2, 7 min after application of E2, 8 min after application of 8 mm glucose, and 3 min after application of 100 μm diazoxide. B, Percentage of activity of the KATP channels elicited by vehicle (control) (n =13), 1 nm E2 (n =5), and 8 mm glucose (n =4). C, 1 nm E2 had no significant effect on KATP channel activity in intact pancreatic islet cells from GC-A KO mice. As in A, the records show KATP channel activity before application of E2, 7 min after application of E2, 8 min after application of 8 mm glucose, and 3 min after application of 100 μm diazoxide. D, Percentage of activity of the KATP channels elicited by vehicle (n =8), 1 nm E2 (n =8), and 8 mm glucose (n =3). **, P < 0.01 Student’s t test comparing 8 mm glucose with control.