Table 2.

HDLs increase the proportion of the γ−form of 4E-BP1 in β−cells experiencing ER stress

	No HDL	With HDLs	Variation induced by HDLs	No. of independent experiments
Serum deprivation	47.9 ± 5.1%	52.8 ± 4.7%	+4.9 ± 1.7%a	10
IL-1β	52.1 ± 2.2%	54.2 ± 1.9%	+2.1 ± 0.5%a	6
TM	50.4 ± 4.9%	62.6 ± 5.0%	+12.2 ± 3.7%a	5
TG	57.5 ± 1.9%	70.9 ± 5.2%	+16.2 ± 2.7%a	3

The percentage of the γ form of 4E-BP1 among total 4E-BP1 in cells subjected to the indicated stresses in the absence or in the presence of HDLs was determined from Western blot analyses such as those depicted in Figs. 3, 4, and 6. The variation of this percentage induced by HDLs is indicated in the last column (^a indicates a statistically significant increase as assessed by t test analyses after Bonferonni correction). The increase in the variation induced by HDLs in cells subjected to ER stress (TM or TG) was significantly higher (∼3- to 6-fold) than the corresponding increase in cells exposed to serum deprivation or IL-1β (P < 0.001; one-way ANOVA analysis). In contrast, the increase in the variation induced by HDLs was not statistically different within the “serum deprivation/IL-1β” group or within the “ER stress” group (P = 0.26).