Fig. 1.

Generation and initial characterization of the ERα^EAAE mutant. The location of the four mutated amino acids in the first zinc finger of the ERα (Y₂₀₁ was changed to E, K₂₁₀ to A, K₂₁₄ to A, R₂₁₅ to E), hence the name EAAE, is indicated (A). In Hela cells, the mutated ERα is unable to activate a luciferase reporter with 2 ERE neither with nor without 10⁻⁸ m E2, but the mutated ERα can still repress a NF-κB-responsive promoter (ICAM-tk-Luc with three NF-κB sites) in an E2-dependent manner (B). Female homozygous ERα^EAAE mice develop hypoplastic uteri (indicated by an arrow) and have blunted mammary gland development (C). In nuclear protein extracts from the livers of wild-type and EAAE mice, the ERα protein is present, but not in liver-specific ERα knockout mice (31 ) (D). Ctrl, Control.