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EGF and TGFα induce EGFR phosphorylation and Erk1/2 activation in Ishikawa and MCF7-TR1 cells. Phosphorylation of EGFR Tyr1045 and Tyr1173 sites (A and B) and ERK1/2 (C) after a rapid treatment (5 min) with 100 ng/ml EGF or TGFα in Ishikawa and MCF7-TR1 cells. Total EGFR and ERK2 levels were used as a loading control. Data shown are representative of three independent experiments.
