Fig. 6.

EGF and TGFα up-regulate c-fos protein levels in Ishikawa and MCF7-TR1 cells. Immunoblots of c-fos from cells treated for 2 h with vehicle (−), 100 ng/ml EGF, or 100 ng/ml TGFα alone and in combination with 10 μm of the EGFR inhibitor tyrphostin AG, the MEK inhibitor PD (A and B), or by silencing EGFR expression (C and D). The efficacy of EGFR silencing was evaluated by immunoblots as indicated. β-Actin served as a loading control. Data shown are representative of three independent experiments.