Fig. 4.

MiR-132 inhibitor partially blocks IL-8 and MCP-1 production. Human preadipocytes were transfected for 24 h in DMEM/F12 containing 10% FBS with varying concentrations of miR-132 inhibitor or a scrambled oligonucleotide negative control (500 nm). Cells were then incubated with fresh serum-containing medium or with serum-free medium for 24 h as indicated. After incubation, medium was collected, and the quantity of IL-8 (A) and MCP-1 (B) was determined using MSD assay kits. Proteins were quantitated by electrochemiluminescence detection and a MSD SECTOR Imager 6000. Data are expressed as light units of IL-8 and MCP-1 and represent the mean of eight replicates ± sem. These data are representative of three independent experiments.