Fig. 7.
miR-132 mediates translational repression of SirT1 through a binding site in SirT1 3′-UTR. Human preadipocytes (1 × 105 cells) were plated in 96-well plates and cotransfected with 200 ng pMIR-Report luciferase vector including the 3′-UTR of SirT1 containing the predicted miR-132 binding site and pre-miR-132 or scrambled oligonucleotide using an electroporator. Luciferase assays were performed using the dual-luciferase assay system 6–8 h after transfection. Data represent the mean of four biological replicates. The data are representative of two independent experiments.