MBX-102 acid enhances insulin sensitivity in vitro in 3T3-L1 adipocytes. Rosiglitazone and MBX-102 acid enhanced glucose uptake activity at submaximal concentration of insulin (0.05 nm) in 3T3-L1 adipocytes (A, Scramble). Silencing of PPAR-γ with P17 siRNA duplexes, as assessed at the protein (B) and message levels (C), fully abolished enhancement by both PPAR-γ agonists (A, PPAR-γ P17). In contrast, enhancement of basal glucose transport by ET-1 was not abolished in PPAR-γ-deficient adipocytes (A, PPAR-γ P17), indicating the cells were able to properly respond to non-PPAR-γ stimuli. Values represent mean ± sem (n = 4; **, P < 0.001 vs. DMSO-treated adipocytes, two-way ANOVA and Bonferroni multiple comparison test). FC, Fold change.