Fig. 2.

GnRHR siRNA inhibits GnRH I-induced trophoblastic cell invasion. A, JEG-3 cells were transiently transfected with scramble siRNA or GnRHR siRNA, and the expression of GnRHR was determined by Western blot with specific antibody. B, JEG-3 cells were transiently transfected with specific GnRHR siRNA or scramble siRNA. Transwell invasion assay was performed in the cells treated with GnRH I (100 nm) or GnRH II (100 nm). The data derived from at least three separate sets of experiments were standardized to the corresponding control, and the statistical results are presented in the column graphs. a, P < 0.05 vs. control cells transfected with scramble siRNA; b, P < 0.05 vs. GnRH I-treated cells transfected with scramble siRNA.