Fig. 4.

EGFR transactivation mediates the activation of ERK and JNK as well as cell invasion induced by GnRH II, but not by GnRH I, in EVTs. A and B, The EVTs that were serum starved as described were stimulated with GnRH I or GnRH II (100 nm, 10 min) in the presence or absence of 30-min pretreatment with vehicle (0.1% DMSO) or AG1478 (5 μm, an EGFR inhibitor). The phosphorylation of ERK1/2 (A) or JNK (B) was determined by Western blotting with specific antibodies. C, Invasive capacity of EVTs that were treated with GnRH I or GnRH II (100 nm), in the presence or absence of pretreatment with vehicle (0.1% DMSO) or AG1478 (5 μm), was measured by invasion assay. The data derived from at least three separate sets of experiments were standardized to the corresponding control, and the statistical results are presented in the column graphs. a, P < 0.05 vs. control; b, P < 0.05 vs. treatment with GnRH II alone; c, P < 0.05 vs. treatment with GnRH II alone.