Fig. 5.

Transactivation of EGFR by GnRH II. A, The EVTs that were serum starved as described were stimulated with GnRH I or GnRH II (100 nm, 10 min). B, After 30-min pretreatment with Antide (100 nm, a GnRHR antagonist), the EVTs were stimulated with GnRH II (100 nm, 10 min). The phosphorylation of EGFR was determined by Western blotting with specific antibody. The data derived from at least three separate sets of experiments were standardized to the corresponding control, and the statistical results are presented in the column graphs. a, P < 0.05 vs. control.