Fig. 6.

Effects of GRK2, GRK3, GRK5, or GRK6 depletion on OTR desensitization in primary myometrial cells. Cells were cotransfected with eGFP-PH and either anti-GRK2 (10 nm), anti-GRK3 (100 nm), anti-GRK5 (100 nm), anti-GRK6 (10 nm), or negative control siRNA (100 nm). After 48 h cells were subjected to the standard (R1, R2 = 30 sec, with 5-min washout between oxytocin additions) OTR desensitization protocol. Representative images and traces showing effects of negative control (A), anti-GRK2 (B), anti-GRK3 (C), anti-GRK5 (D), and anti-GRK6 (E) on OTR desensitization. Cumulative data (F) show that depletion of endogenous GRK6 significantly (P < 0.01) prevents OTR desensitization. Data are shown as means ± sem for the % change in R2:R1 ratio for between eight and 15 cells from at least eight separate experiments for each treatment group. Data were obtained from cells prepared from at least three different patient donors. **, P < 0.01 comparing anti-GRK6 siRNA treatment with respective negative-control siRNA, anti-GRK2, anti-GRK3, and anti-GRK5 treatments. NC, Negative control.