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. 1990 Jun;87(12):4737–4741. doi: 10.1073/pnas.87.12.4737

Functional expression of murine multidrug resistance in Xenopus laevis oocytes.

G Castillo 1, J C Vera 1, C P Yang 1, S B Horwitz 1, O M Rosen 1
PMCID: PMC54192  PMID: 1693776

Abstract

The development of multidrug resistance (MDR) is associated with the overproduction of a plasma membrane glycoprotein, P glycoprotein. Here we report the functional expression of a member of the murine mdr family of proteins and show that Xenopus oocytes injected with RNA encoding the mouse mdr1b P glycoprotein develop a MDR-like phenotype. Immunological analysis indicated that oocytes injected with the mdr1b RNA synthesized a protein with the size and immunological characteristics of the mouse mdr1b P glycoprotein. These oocytes exhibited a decreased accumulation of [3H]vinblastine and showed an increased capacity to extrude the drug compared to control oocytes not expressing the P glycoprotein. In addition, competition experiments indicated that verapamil, vincristine, daunomycin, and quinidine, but not colchicine, can overcome the rapid drug efflux conferred by the expression of the mouse P glycoprotein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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