Figure 9. Estimation of the optimum reaction conditions for PL.

The effect of (A) reaction temperature, (B) reaction pH on PL enzymatic activity (U/mL), (C) substrate concentration, and (D) enzyme concentration on reaction speed (10⁻⁵ mmol/l/g), and the addition order of reagents on PL activity inhibition rate (%) (1: substrate and enzyme were prewarmed at 37°C for 10 min first, and then the inhibitor was added; 2: substrate and inhibitor were prewarmed at 37°C for 10 min first,and then the substrate was added) were determined by colorimetric methods using pNPB) as a chromogenic substrate. Data are expressed as the mean ± SD from triplicate experiments. Different small letters next to values indicate significant differences (p < 0.05).