Fig. 1.

Oocyte calcein uptake through GJC during oocyte maturation. A, After calcein-AM uptake by the COC, calcein fluorescence was measured in the oocyte after various times of IVM. B, After calcein measurement, meiotic resumption in the same oocytes was assessed by detection of GVBD. C, The effect on oocyte calcein fluorescence by exposing COC to the GJC inhibitor carbenoxolone (100 μm) before and during calcein transfer. D, The effect of gonadotropins treatment during IVM on oocyte calcein uptake. GV, Germinal vesicle; PMSG, pregnant mare serum gonadotropin.