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. 2009 May;23(5):700–710. doi: 10.1210/me.2008-0320

Fig. 5.

Fig. 5.

Modulation of DRM composition and association with Cx43 during IVM. Sucrose density gradient centrifugation was performed on freshly isolated COCs (A and B, first panel) and COCs recovered after 4 h (A and B, second panel) and 24 h of IVM with gonadotropins (A and B, third panel). Dot blot and hybridization with CTB revealed GM1 enrichment in the DRM-containing fractions of each preparation (A). Hybridization with an anti-Cx43 antibody revealed an increase in DRM-associated Cx43 as IVM progressed (B). Alkaline phosphatase treatment of fraction 4 from gradients performed after 4 and 24 h of IVM revealed that DRM-associated Cx43 is mostly in phosphorylated form (C). The values presented under each Cx43 panel represent the percentage of Cx43 distributed in the different fraction groups. AP, Alkaline phosphatase; Ctrl, control; IB, immunoblotting; I, intermediate fractions; NR, non-raft fractions; GNT, gonadotropins.