Fig. 8.

Impact of gonadotropins on DRM-associated Cx43. Sucrose density gradient centrifugation was performed on COCs recovered after 24 h of IVM without gonadotropins. GM1 and Cx43 detection was performed on each fraction (A and B). Fraction 4 samples from gradients performed on COCs after 24 h of IVM with and without gonadotropins were analyzed by Western blotting with an anti-Cx43 antibody. Higher DRM-associated Cx43 in gonadotropin-treated COCs are observed compared with untreated COCs (C). Treatment of the same fractions with alkaline phosphatase (AP) revealed that DRM-associated Cx43s are mostly phosphorylated. IB, Immunoblotting; NR, non-raft fractions; I, intermediate fractions.