Fig. 7.
TSHr down-regulation is not the crucial event in Ras oncogene-induced dedifferentiation. A, Schematic representation of SPRT-TSHr-expressing clones, which keep the expression of the ectopic TSHr (hTSHr) even after ER-RasV12 activation. B and C, Cl11- and SPRT-TSHr-expressing clones were treated 24 h with or without 4OHT. B, Total proteins were analyzed by Western blot for the reported genes. In T4 and T17 clones the ectopic hTSHr is identified by the band migrating above the endogenous rTSHr visible in the untreated Cl11. C, cAMP was quantitatively measured in each clone before and after the 4OHT treatment. **, P < 0.01.