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IL-6 treatment represses forskolin-induced expression of G6Pase in HepG2 cells. HepG2 cells were placed in OptiMEM for 16 h and then treated with IL-6 (20 ng/ml) or forskolin (10 μm) or both for the indicated time, and RNA was extracted. qPCR analysis was performed for SOCS3 expression (A) and G6Pase expression (B). Error bars represent ± sd of the mean; *, P ≤ 0.05.
