Fig. 4.

IL-6 activates STAT3 in mouse liver, induces SOCS3 expression, and represses G6Pase expression. C57BL6 mice (10 wk of age) were fasted for approximately 16 h and injected ip with PBS or 100 ng of IL-6. Mice were euthanized at the indicated times after injection (panel B, minutes after injection; panels C–I, 1 h after injection) and livers were dissected. Lysates were prepared and analyzed by Western blot for P-STAT3 and total STAT3 levels (A). RNA was extracted and qPCR analysis for SOCS3 and G6Pase was performed (B) (n = 2). A similar experiment with n = 8 per group was performed, and qPCR analysis was performed for SOCS3, G6Pase, PEPCK, FAS, PGC1α, and SREBP1c expression (C–H). Blood glucose levels were measured before and after injections before euthanizing the mice (I). Error bars represent ± sem of the mean; *, P ≤ 0.05.