Fig. 5.

Repression of G6Pase by IL-6 requires STAT3 function in vivo. C57BL/6 mice (n = 8–11 mice per group) were injected with Ad-GFP control or Ad-GFP STAT3β through the tail vein on d 1. Mice were fasted overnight on d 4, and on d 5 were injected ip with PBS or 100 ng of IL-6 and euthanized 1 h after injection, after which livers were collected. Western blot analyses for P-STAT3 and total STAT3 from a representative group of mice (A) and qPCR analysis for SOCS3, G6Pase, and PEPCK mRNA levels (B–D) are shown. Error bars represent ± sem of the mean; *, P ≤ 0.05.