Decrease in acetylated histone H4 and RNA Pol II recruitment is seen at the G6Pase promoter in response to IL-6 treatment. ChIP assays were performed to detect the presence of acetylated histone H4, acetylated histone H3, and RNA Pol II at the SOCS3 (A), G6Pase (B), and PEPCK (C) promoters. A control PCR for the β-globin promoter was also performed. Regions where changes in acetylated histone H4 and RNA Pol II were seen at the G6Pase promoter are underlined. Quantified band intensities from panels A, B, and C are shown graphically (D). Ab, Antibody; TSS, transcription start site.