Fig. 2.

Comparison of the transcriptional activity of HA-PPARγ2 and HA-PPARγCDE on genomic target genes. NIH-CAR cells were transduced with AdEmpty (AdE), AdHA-PPARγ2 (AdHA-γ2), or AdHA-PPARγCDE (AdHA-γCDE) and treated with vehicle (DMSO), rosiglitazone (Rosi), or antagonist GW9662 (GW) as indicated. RNA was isolated 8 h after transduction, and mRNA expression was determined by RT-qPCR and normalized to the corresponding TFIIB levels. Results are representative of at least three independent experiments.