Fig. 3.

Comparison of the transcriptional activity of HA-PPARγ2, HA-PPARγCDE, and HA-PPARαA/BγCDE on genomic target genes. A, Schematic diagram of the HA-tagged PPARα and -γ and the chimeric PPARαA/BγCDE encoded by the recombinant adenoviruses. Numbers indicate amino acid positions of domain borders (Swiss-Prot; www.expasy.org). B–D, NIH-CAR cells were transduced with AdEmpty (AdE), AdHA-PPARγ2 (AdHA-γ2), AdHA-PPARγCDE (AdHA-γCDE), or AdHA-PPARαA/BγCDE (AdHA-αA/BγCDE) and treated with vehicle (DMSO) or rosiglitazone (Rosi) as indicated. The cells were harvested 8 h after transduction and subjected to either SDS-PAGE and immunoblotted using antibodies against PPARγ and TFIIB or RNA purification. mRNA expression was determined by RT-qPCR and normalized to the corresponding TFIIB levels. Results are representative of at least three independent experiments.