Fig. 4.

The effect of A/B-domain deletion on the lipogenic potential of HA-PPARγ2. NIH-CAR cells were transduced with AdEmpty (AdE) and treated with vehicle (DMSO) or transduced with AdHA-PPARγ2 (AdHA-γ2) or AdHA-PPARγCDE (AdHA-γCDE) and treated with rosiglitazone (Rosi). A, Cells were harvested 48 h after transduction and subjected to SDS-PAGE and immunoblotted using antibodies against PPARγ and TFIIB. B, Lipid synthesis was assessed in triplicate by determining incorporation of [¹⁴C]acetate into total lipids 48 h after adenoviral transduction of which the last 24 h were in the presence of [¹⁴C]acetate. Extracted lipids were separated on silica thin-layer chromatography plates and radioactivity in the triglyceride fraction was quantified. Results are representative of two independent experiments.