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. 2009 Jun;23(6):794–808. doi: 10.1210/me.2008-0236

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Fig. 5. — HA-PPARγ2:RXR and HA-PPARγCDE:RXR heterodimers bind equally well to most genomic PPREs. Cross-linked chromatin was harvested from NIH-CAR cells 8 h after transduction with either AdEmpty (AdE) and treatment with vehicle (DMSO) or transduction with AdHA-PPARγ2 (AdHA-γ2) or AdHA-PPARγCDE (AdHA-γCDE) and treatment with rosiglitazone (Rosi). Relative occupancy at PPREs of interest was determined by ChIP-qPCR using antibodies against HA and RXR, respectively. Results are representative of at least three independent experiments. The figure shows means of two experiments with indication of range.