Fig. 8.
HA-PPARγ2 and HA-PPARγCDE differ in their ability to recruit CBP and p300 occupancy to genomic PPREs. Cross-linked chromatin was harvested from NIH-CAR cells 8 h after transduction with either AdEmpty (AdE) and treatment with vehicle (DMSO) or transduction with AdHA-PPARγ2 (AdHA-γ2) or AdHA-PPARγCDE (AdHA-γCDE) and treatment with rosiglitazone (Rosi). Relative occupancy at PPREs of interest was determined by ChIP-qPCR using antibodies against CBP or p300, respectively. Results are representative of at least three independent experiments. The figure shows means of two experiments with indication of range.