Table 1A.
Continued
| Gene | Accession no. | HA-PPARγ2 + Rosi, fold induction | HA-PPARyCDE + Rosi, fold induction | HA-PPARγ2 vs. HA-PPARγCDE, fold change | P value |
|---|---|---|---|---|---|
| Growth/proliferation | |||||
| BTG1 | L16846 | 1.54 | 1.40 | 1.10 | 0.060 |
| BTG2 | NM_007570 | 5.35 | 3.11 | 1.72 | 0.053 |
| CDC25A | C76119 | 1.52 | 1.37 | 1.11 | 0.249 |
| CDK2AP2 | NM_026373 | 1.98 | 1.83 | 1.08 | 0.446 |
| CUL1 | BG070097 | 1.59 | 1.39 | 1.15 | 0.287 |
| CYR61 | BB533736 | 1.86 | 1.77 | 1.05 | 0.761 |
| EEF2K | C86191 | 1.51 | 1.38 | 1.09 | 0.287 |
| EPS8 | BM213788 | 1.72 | 1.52 | 1.13 | 0.529 |
| ERBB2IP | BB336138 | 1.84 | 1.82 | 1.01 | 0.836 |
| FGFRLI | AF321301 | 2.93 | 1.98 | 1.49 | 0.014 |
| FOSL11 | U34245 | 1.65 | 1.49 | 1.10 | 0.322 |
| GSPT1 | BB162021 | 1.59 | 1.24 | 1.28 | 0.154 |
| KLF10 | NM_013692 | 1.69 | 1.68 | 1.01 | 0.893 |
| NEK6 | BB528391 | 1.97 | 1.86 | 1.06 | 0.166 |
| NET1 | NM_019671 | 1.65 | 1.56 | 1.06 | 0.311 |
| NRP1 | AK011144 | 1.65 | 1.49 | 1.10 | 0.073 |
| NRP2 | BB409477 | 1.65 | 1.46 | 1.13 | 0.144 |
| P8 | NM_019738 | 1.60 | 1.27 | 1.25 | 0.106 |
| PFTK1 | AI327038 | 1.61 | 1.44 | 1.12 | 0.164 |
| PLK32 | BM947855 | 2.28 | 1.45 | 1.57 | 0.048 |
| POLD4 | AK010477 | 1.55 | 1.45 | 1.06 | 0.338 |
| RALGDS | NM_009058 | 1.61 | 1.47 | 1.09 | 0.302 |
| RBPJ | NM_009035 | 1.53 | 1.52 | 1.01 | 0.816 |
| RRM2 | NM_009104 | 1.73 | 1.57 | 1.11 | 0.259 |
| SPRY42 | BB080456 | 2.63 | 1.55 | 1.70 | 0.018 |
| STK1O | NM_009288 | 2.01 | 1.81 | 1.11 | 0.039 |
| TNK2 | NM_016788 | 1.63 | 1.17 | 1.39 | 0.097 |
| TOB1 | BQ266486 | 2.68 | 1.91 | 1.41 | 0.016 |
| TRIM25 | D63902 | 2.99 | 2.67 | 1.12 | 0.114 |
| VEGFC C | NM_009506 | 2.78 | 2.15 | 1.30 | 0.135 |
The table presents clustered genes induced more than 1.5-fold in NIH-CAR cells transduced with either AdHA-PPARγ2 or AdHA-PPARγCDE in the presence of rosiglitazone (Rosi). Background is defined as cells transduced with AdEmpty in the presence of vehicle (DMSO). The mRNA levels were detected by Affymetrix microarray analysis (GEO accession no. GSE15433) or RT-qPCR (indicated) performed in triplicate on independent biological experiments. Fold change in expression level between cells transduced with AdHA-PPARγ2 relative to cells transduced with AdHA-PPARγCDE and the corresponding P value are indicated. For the genes detected by RT-qPCR, a representative fold change is indicated.
Genes registered as regulated by PPARγ or PPARγ agonists in the Ingenuity database.
A/B-domain-dependent genes, i.e. genes displaying significantly reduced induction in the cells expressing HA-PPARγCDE.