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Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1990 Jun;87(12):4742–4745. doi: 10.1073/pnas.87.12.4742

Preincubation in acid medium increases Na/H antiporter activity in cultured renal proximal tubule cells.

S Horie 1, O Moe 1, A Tejedor 1, R J Alpern 1
PMCID: PMC54193  PMID: 2162055

Abstract

Chronic acidosis in vivo leads to an increase in proximal tubule Na/H antiporter activity that persists when the transporter is studied out of the acidotic environment. It is presently not clear whether a decrease in extracellular fluid pH alone is sufficient to elicit this adaptation. The present studies examined the effect of acid preincubation on Na/H antiporter activity in cultured proximal tubule cells. Antiporter activity was examined after a 2-day preincubation in control or acid medium, 1 hr after removal from the preincubation fluid. Na/H antiporter activity was assayed as the initial rate of Na-dependent alkalinization after cell acidification in the absence of CO2/HCO3. Preincubation in low [HCO3] acid medium or in high PCO2 acid medium led to increases in amiloride-sensitive Na/H antiporter activity. This adaptation was inhibited by addition of cycloheximide to the preincubation medium. Preincubation of fibroblasts in low [HCO3] acid medium did not lead to increased Na/H antiporter activity but rather caused a small inhibition. These studies demonstrate an adaptation in Na/H antiporter activity elicited by a low pH of the extracellular fluid, which is dependent on protein synthesis, and may be unique to certain H/HCO3-transporting epithelia.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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