Fig. 1.

CART Regulation of FSH-Induced Erk1/2 Signaling

Time course of the relative increase in Erk1/2 activation (pErk/total Erk) vs. time zero observed for bovine GCs collected after stimulation with 25 ng/ml FSH, 25 ng/ml FSH preceded by 24 h preincubation in the presence of 0.1 μm CART, or after costimulation with FSH + CART (A). Representative Western blots demonstrating the pattern of Erk1/2 activation in GCs stimulated with FSH alone (B), FSH + CART; vehicle was water (C) or subjected to 24 h CART pretreatment followed by FSH stimulation (D). The data depicted in panel A are represented as the mean ± sem from three replicate experiments and different superscripts denote significant differences across and within treatments and time points (P < 0.05).