Fig. 13.

CART Regulation of Proteasomal Degradation of DUSP5 and PP2A

Representative Western blots demonstrating the effect of treatment of bovine GCs with the proteasome inhibitor Z-LLF-CHO (10 μm) on FSH (25 ng/ml) and FSH + CART (0.1 μm)-induced protein expression for DUSP5 (A) and PP2A-catalytic subunit (PP2A) (B) and on Erk1/2 activation (pErk/total Erk) (C). Relative increase in GC expression of DUSP5 (D) and PP2A (E) proteins vs. time zero observed in response to above treatments. The data depicted in panels D and E are represented as the mean ± sem from three replicate experiments, and different superscripts denote significant differences across treatments (P < 0.05).