Fig. 14.

CART Regulation of Accumulation of Polyubiquitinated Proteins

Representative Western blot for polyubiquitinated proteins in cell lysates from bovine GCs stimulated with FSH (25 ng/ml) for 0 and 30 min, CART (0.1 μm) for 60 min, FSH + CART for 30 and 60 min, or preincubated with the proteasome inhibitor, Z-LLF-CHO (10 μm) for 240 min followed by stimulation with FSH for 30 min. Polyubiquitinated proteins detected appear as a smear. Corresponding Western blot for actin was used as a loading control.