Fig. 2.

CART Regulation of FSH-Stimulated Akt Activation

Time course of the relative increase in Akt activation (pAkt-Ser⁴⁷³/total Akt) vs. time zero observed for bovine GCs collected after stimulation with 25 ng/ml FSH, 25 ng/ml FSH preceded by 24 preincubation in the presence of 0.1 μm CART, or after costimulation with FSH + CART (A). Representative Western blots demonstrating the pattern of Akt activation in GCs stimulated with FSH alone (B), FSH + CART; vehicle was water (C) or subjected to 24 h CART pretreatment followed by FSH stimulation (D). The data depicted in panel A are represented as the mean ± sem from three replicate experiments, and different superscripts denote significant differences across and within treatments and time points (P < 0.05).