Fig. 4.

Signaling Pathways Involved in CART-Induced Erk1/2 Activation

Relative decrease in Erk1/2 activation (pErk/total Erk) vs. media alone control observed for bovine GCs preincubated in the presence of 1 μm of different signaling protein inhibitors (PKC, PKA, PI3K, G_o/i, MEK, MEK1/2, and RTK) for 24 h followed by 15 min CART (0.1 μm) stimulation (A). Representative Western blots demonstrating the pattern of Erk1/2 activation in GCs treated with media containing equivalent volume of DMSO (vehicle) or with above signaling protein inhibitors for 24 h before CART stimulation (B). The data depicted in panel A are represented as the mean ± sem from three replicate experiments, and different superscripts denote significant differences between treatments (P < 0.05).