Fig. 8.

CART-Induced Expression of DUSP5 and PP2A

Representative Western blots demonstrating levels of DUSP5 in bovine GCs stimulated with FSH (25 ng/ml) for 0, 30, and 120 min, CART (0.1 μm) for 60 min, or FSH + CART for 30 and 120 min (A). Relative increase in expression of DUSP5 vs. time zero observed in response to above treatments (B). Representative Western blots demonstrating levels of PP2A-catalytic subunit (PP2A) in GCs stimulated with FSH (25 ng/ml) for 0, 30, and 120 min, CART (0.1 μm) for 60 min, or FSH + CART for 30 and 120 min (C). Relative increase in expression of PP2A vs. time zero observed in response to above treatments (D). The data in panels B and D are represented as the mean ± sem from three replicate experiments, and different superscripts denote significant differences between treatments (P < 0.05).