Fig. 9.

Signaling Pathways Involved in CART-Induced Expression of DUSP5 and PP2A

Representative Western blots demonstrating levels of DUSP5 and PP2A-catalytic subunit (PP2A) in bovine GCs preincubated with media alone or with 1 μm MEK1/2 inhibitor (U0126) or 1 μm PKC peptide inhibitor 19–27 for 24 h before stimulation with FSH (25 ng/ml) for 0 and 30 min, CART (0.1 μm) for 60 min, or FSH + CART for 30 min (A). Relative increase in GC expression of DUSP5 (B) and PP2A (C) vs. time zero observed in response to above treatments. The data depicted in panels B and C are represented as the mean ± sem from three replicate experiments. Different superscripts denote significant differences between treatments (P < 0.05).