Fig. 6.

Expression of MyoD, Myogenin, and p21 in Response to Ligand Treatment

C₂C₁₂ cells were cultured in DM (2% horse serum) for 96 h in the presence (+) or absence (−) of indicated ligands, added at 8 μg/ml. Cell lysates were run on a reducing SDS-PAGE gel and immunoblotted for p21, myoD, myogenin, and β-actin proteins. Both myogenin and p21 were down-regulated by the treatment of all ligands, with the exception of activin A. In contrast, the levels of MyoD were down-regulated only when treated with BMP-9 and -10.