. 2008 Dec;22(12):2689–2702. doi: 10.1210/me.2008-0290

Table 1.

TGF-β Family Members Identified in ActRIIB-Fc Pulldowns from Mouse and Human Sera

GI No.¹	Protein Name	% Sequence Coverage²	Sequence	z³	X_corr⁴
Mouse serum
111494000	Myostatin	45	(R)DFGLDCDEHSTESR	2+	4.52
			(K)ANYCSGECEFVFLQK	2+	4.49
			(K)M* SPINM* LYFNGK⁵	2+	3.37
			(K)GSAGPCCTPTK(M)	2+	2.77
			(K)IPAM* VVDR	2+	2.48
4160597	BMP-11	67	(R)NLGLDCDEHSSESR	2+	4.72
			(K)ANYCSGQCEYM* FM* QK	2+	4.58
			(K)M* SPINM* LYFNDK	2+	3.54
			(K)YPHTHLVQQANPR	3+	3.51
			(R)GSAGPCCTPTK	2+	3.14
			(K)IPGMVVDR	2+	2.18
			(K)IPGM* VVDR	2+	2.11
50148	Inhibin β_B	38	(K)LSSM* SM* LYFDDEYNIVKR	3+	2.94
			(R)GLNPGPVNSCCIPTK	2+	2.90
			(R)GLNPGPVNSCC#IPTK⁶	2+	2.87
			(R)DVPNM* IVEECGCA	2+	3.00
74184431	TGF-β1	21	(−)ALDTNYCFSSTEK	2+	3.84
			(K)VEQLSNMIVR	2+	3.43
			(K)VEQLSNM* IVR	2+	3.07
5932440	BMP-9	28	(R)VNFEDIGWDSWIIAPK	3+	4.09
			(K)GGCFFPLADDVTPTK	2+	3.53
3873289	BMP-10	29	(K)LDPISILYLDK	2+	2.78
			(K)YEGM* AVSECGCR	2+	2.36
			(K)TPLYIDFK	2+	2.35
Human serum
4885259	Myostatin	39	(K)ANYCSGECEFVFLQK	2+	4.40
			DFGLDCDEHSTESR	3+	2.60
			M* SPINMLYFNGK	2+	2.70
			GSAGPCCTPTK	2+	2.40
6649914	BMP-11	35	(K)M* SPINM* LYFNDK	2+	2.40
			(R)GSAGPCCTPTK	2+	2.40
			(R)RNLGLDCDEHSSESR	3+	2.60
186417	Inhibin β_B	39	(R)GLNPGTVNSCCIPTK	2+	3.30
			(R)DVPNM* IVEECGCA	2+	2.20
			(K)LSTM* SM* LYFDDEYNIVK	2+	4.70
755740	Inhibin β_A	24	(K)DIQNM* IVEECGCS	2+	3.84
			(K)QFFVSFK	2+	2.30
			(K)KQFFVSFK	2+	3.10
			(K)SCCVPTK	2+	2.04
22761274	Inhibin β_E	38	(K)ANNPWPASTSCCVPTAR	2+	3.50
			(K)TDVPDM* VVEACGCS	2+	2.80
			(R)TPTCEPATPLCCR	2+	3.60
5031795	Inhibin β_C	61	(R)AGGQCPACGGPTLELESQR	2+	5.20
			(K)ANTAAGTTGGGSCCVPTAR	2+	5.00
			(R)GIDCQGGSR	2+	2.90
			(R)RPLSLLYYDRDSNIVK	3+	3.10
			(R)QEFFVDFR	2+	2.10
67463993	BMP-9⁷	14	(K)GGCFFPLADDVTPTK	2+	4.90
75516754	BMP-10	35	(K)ACCVPTKLEPISILYLDK	3+	4.15
			(K)LEPISILYLDK	2+	2.84
			(R)TPLYIDFK	2+	2.66
			(K)YEGM* AVSECGCR	2+	3.30

^¹

GI No., GenInfo Identifier, used to access protein sequence records in NCBI.

^²

Percent sequence coverage represents the percent of the protein amino acid sequence that can be mapped by the identified peptides from MS.

^³

z is the charged state of the parent peptide ion.

^⁴

X_corr is the Sequest search cross-correlation value. It increases with peptide length and charge.

^⁵

M*, Methionine oxidation.

^⁶

C#, Iodoacetamide modification of cysteine.

^⁷

Annotated fragment ion spectrum of this peptide is shown in Supplemental Fig. 1a.