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Phosphorylation of Ser-318 Is Not Necessary for Phosphorylation of Ser-307
A and B, C2C12 cells transfected with IRS-1 wt, IRS-1 A302/318, or IRS-1 E318 were stimulated with 10 nm insulin as indicated. Shown are representative immunoblots for phospho-Ser-307 and the corresponding reprobe for IRS-1 protein and in the lower part the densitometric quantification (n = 4, mean ± sem). ins, Insulin; rel., relative.
