Fig. 1.

DEX Resistance of atp1a1 and scnn1a Not in 293 Cells but in HepG2 Cells

A, 293 cells and HepG2 cells were cultured in phenol red-free Opti-MEM I for 24 h and treated with or without 100 nm DEX for 6 h in the presence or absence of 100 nm TSA, as indicated. Total RNA was prepared and endogenous mRNA for Na⁺, K⁺-ATPase α1 (atp1a1), amiloride-sensitive Na⁺ channel 1α (scnn1a), serum and glucocorticoid-regulated kinase 1 (sgk1), alcohol dehydrogenase 1A (adh1a), and glyceraldehyde-3-phosphate dehydrogenase (gapdh) was measured with qRT-PCR. Samples were normalized to gapdh mRNA levels, and relative expression levels to vehicle-treated samples are presented as relative mRNA expression. Error bars represent sd values of at least three independent experiments. B, Cell lysates from 293 cells and HepG2 cells were subjected to Western blot analysis using indicated antibodies.