Fig. 5.

Knockdown of HEXIM1 Enhanced P-TEFb-Dependent Gene Expression

A, HepG2 cells were infected with Adsictrl or AdsiHEXIM1 as described in Fig. 3B and stimulated with 10 nm 3MC, 100 ng/ml IL-6, or culture at 42 C (heat shock) for 1 h as indicated. Endogenous mRNA for cytochrome P450, family 1, subfamily A, polypeptide 1 (cyp1a1), JunB (junB), heat shock 70-kDa protein 1A (hspa1a), Fos (fos), and gapdh was measured with qRT-PCR. Samples were normalized to gapdh, and relative expression levels to the Adsictrl-infected and unstimulated samples are presented as relative mRNA expression. Error bars represent sd values of at least three independent experiments. B, HepG2 cells were infected with the recombinant adenoviruses and stimulated as described in panel A. Nuclear extracts were prepared, and protein expression levels of endogenous AhR, STAT3, HSF1, CDK9, and α-actinin were assessed by Western blotting.