Fig. 3.

Kinetics of CREB Phosphorylation within Cumulus Cells of COCs in Response to FSH or N⁶-cAMP Plus 8-Br-cAMP and the Effect of KG-501 on Oocyte Maturation

Cumulus cells from 50 COCs were collected at various time point for immunoblotting with anti-phos CREB and anti-CREB antibodies.COCs were cultured in HX medium with or without 50 IU/liter FSH (A). COCs were cultured in HX medium containing 50 IU/liter FSH (B) or pulsing with 250 μm N⁶-cAMP and 8-Br-cAMP for 3 h (C). Different concentrations of KG-501 (0–25 μm) were added when mouse COCs were cultured in HX medium containing 50 IU/liter FSH or pulsing with 250 μm N⁶-cAMP (N⁶) plus 8-Br-cAMP (8-Br). The percentage of GVBD was recorded at 24 h of culture. Columns with different letters were significantly different (P < 0.05) (D). The experiment was performed three times with similar results. pCREB, Phosphorylated CREB.