Fig. 5.
Time-Dependent Changes of CYP51 mRNA and Protein Level within Cumulus Cells of COCs in Response to FSH or N6-cAMP (N6) + 8-Br-cAMP (8-Br)
COCs were treated with 50 IU/liter FSH or pulsed with 250 μm N6-cAMP and 8-Br-cAMP in HX medium. COCs were cultured in HX medium as a control. Sample of cumulus cells at various time points were collected for RT-PCR and immunoblotting. CYP51 mRNA and protein levels induced by FSH (panels A and B) or N6-cAMP plus 8-Br-cAMP (panels C and D) during 0–12 h were analyzed. The densitometric data of CYP51 were normalized according to internal standards (β-actin and GAPDH) to evaluate the relative abundance of the targets. *, P < 0.05, compared with each corresponding group in the control. These results are representative of three experiments.