Fig. 8.

Rev-erbα Negatively Interferes with the Induction of MCP-1 and TNFα Secretion by LPS in LXR-Agonist-Treated Macrophages

A and B, Primary human macrophages were infected with a Rev-erbα adenovirus (ad-Rev-erbα) or a GFP (ad-GFP) expressing control adenovirus and then treated with T0901317 (1 μm) or vehicle (Control) for 48 h. C and D, Primary human macrophages transfected with anti-hRev-erbα or scrambled control siRNA were treated with T0901317 (1 μm) or vehicle (Control) for 48 h. Cells were incubated with LPS (100 ng/ml) during 8 h and MCP-1 (A and C) and TNFα (B and D) secretion were analyzed by ELISA. Results are representative of those obtained from three independent macrophage preparations and are expressed relative to the levels in LPS-stimulated cells set as 1. Each bar is the mean value ± sd of triplicate determinations. Statistically significant differences are indicated (ANOVA/t test; adGFP vs. adRev-erbα or scramble vs. siRNA-Rev-erbα: §, P < 0.05; §§, P < 0.01; §§§, P < 0.001 and control vs. T0901317: **, P < 0.01; ***, P < 0.001). Based on two-way ANOVA analysis, Rev-erbα expression significantly affected induction of cytokine response to LPS after T0901317 treatment.