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Endogenous 11β-HSD1 Is Expressed in A549 Cells and Shows Exclusively Reductase Activity
Representative image of thin-layer chromatography plate showing 11keto-reductase activity (conversion of 200 nm [3H]cortisone to cortisol) but no 11β-dehydrogenase activity (conversion of 200 nm [3H]cortisol to cortisone) in intact A549 cells. Arrows indicate cortisone and cortisol. M indicates medium with [3H]steroid alone (no cells).
